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http://hdl.handle.net/11690/131
2024-03-29T14:30:03ZÁrvores nativas do município de Canoas, Rio Grande do Sul [capítulo de livro]
http://hdl.handle.net/11690/3033
Título: Árvores nativas do município de Canoas, Rio Grande do Sul [capítulo de livro]
Autor(es): Trentin, Bruno Alves; Bordignon, Sérgio Augusto de Loreto
Título: Árvores nativas do município de Canoas, Rio Grande do Sul [capítulo de livro]
Autor(es): Trentin, Bruno Alves; Bordignon, Sérgio Augusto de Loreto2019-01-01T00:00:00ZChemical constituents and Pharmacology properties of Aristolochia triangularis: a south brazilian highly-consumed botanical with multiple bioactivities
http://hdl.handle.net/11690/3032
Título: Chemical constituents and Pharmacology properties of Aristolochia triangularis: a south brazilian highly-consumed botanical with multiple bioactivities
Autor(es): Oliveira, Simone Q.; Kratz, Jardel M.; Chaves, Vitor C.; Guimarães, Tatiana R.; Costa, Danielle T. M.; Dimitrakoudi, Sapfo; Vontzalidou, Argyro; Bordignon, Sergio Augusto de Loreto; Simionato, Cesar P.; Steindel, Mário; Reginatto, Flávio H.; Simões, Cláudia M.O.; Schenkel, Eloir P.
Resumo: Aristolochia triangularis Cham., is one of the most frequently used medicinal plant in Southern
Brazil. Preparations containing the leaves and/or stems are traditionally used as anti-inflammatory,
diuretic, as well as antidote against snakebites. This study screened A. triangularis extracts, fractions
and isolated compounds for different bioactivities. A weak antiproliferative activity against human lung
cancer cell line (A549) was observed only for chloroform fraction obtained from stems (CFstems - CC50:
2.93 µg/mL). Also, a moderate antimicrobial activity against Staphylococcus aureus was detected just for
chloroform fraction obtained from leaves (CFleaves -13-16 mm inhibition zone). Additionally, two semipurified fractions (CFstems-4 and CFleaves-4) selectively inhibited HSV-1 replication (IC50 values of 0.40 and
2.61 µg/mL, respectively), while only CFleaves showed promising results against Leishmania amazonensis.
Fractionation of extracts resulted in the isolation of one neolignan (-) cubebin and one lignan (+) galbacin.
However, these compounds are not responsible for the in vitro bioactivities herein detected. The presence
of aristolochic acid I and aristolochic acid II in the crude ethanol extract of stems (CEEstems) and leaves
(CEEleaves) was also investigated. The HPLC analysis of these extracts did not display any peak with
retention time or UV spectra comparable to aristolochic acids I and II.
Título: Chemical constituents and Pharmacology properties of Aristolochia triangularis: a south brazilian highly-consumed botanical with multiple bioactivities
Autor(es): Oliveira, Simone Q.; Kratz, Jardel M.; Chaves, Vitor C.; Guimarães, Tatiana R.; Costa, Danielle T. M.; Dimitrakoudi, Sapfo; Vontzalidou, Argyro; Bordignon, Sergio Augusto de Loreto; Simionato, Cesar P.; Steindel, Mário; Reginatto, Flávio H.; Simões, Cláudia M.O.; Schenkel, Eloir P.
Resumo: Aristolochia triangularis Cham., is one of the most frequently used medicinal plant in Southern
Brazil. Preparations containing the leaves and/or stems are traditionally used as anti-inflammatory,
diuretic, as well as antidote against snakebites. This study screened A. triangularis extracts, fractions
and isolated compounds for different bioactivities. A weak antiproliferative activity against human lung
cancer cell line (A549) was observed only for chloroform fraction obtained from stems (CFstems - CC50:
2.93 µg/mL). Also, a moderate antimicrobial activity against Staphylococcus aureus was detected just for
chloroform fraction obtained from leaves (CFleaves -13-16 mm inhibition zone). Additionally, two semipurified fractions (CFstems-4 and CFleaves-4) selectively inhibited HSV-1 replication (IC50 values of 0.40 and
2.61 µg/mL, respectively), while only CFleaves showed promising results against Leishmania amazonensis.
Fractionation of extracts resulted in the isolation of one neolignan (-) cubebin and one lignan (+) galbacin.
However, these compounds are not responsible for the in vitro bioactivities herein detected. The presence
of aristolochic acid I and aristolochic acid II in the crude ethanol extract of stems (CEEstems) and leaves
(CEEleaves) was also investigated. The HPLC analysis of these extracts did not display any peak with
retention time or UV spectra comparable to aristolochic acids I and II.2019-01-01T00:00:00ZEx situ method and Salix spp. to treat polluted soil with hydrocarbon
http://hdl.handle.net/11690/3031
Título: Ex situ method and Salix spp. to treat polluted soil with hydrocarbon
Autor(es): Sabedot, Sydney; Bordignon, Sérgio Augusto de Loreto; Cunha, Ana Cristina Borda da
Resumo: This study shows the practices for managing ex situ soil with a simulation in which a residue derived from hydrocarbons with a sandy-silty soil was mixed, and two Salix spp. varieties were planted in the mixture. The experiment took place in a greenhouse and controls on plants and soil occurred during 36 months. The experiment aimed at two objectives, the first of which was to evaluate the development and resistance of the plants in the contaminated soil, comparing them with their planting in sandy-silty soil without the residue, and the second was to evaluate the performance of plants as phytoremediators of the residue. This study deals with the first goal. The success in sprouting and in development of the plants was verified in both types of soils. The diameter and height of the stems and the dimensions of the leaves were the measured parameters. Statistical analysis of the data indicated that Salix triandra species developed better. The joint result for both the above mentioned objectives indicated that the ex situ method for treating soils contaminated with hydrocarbons and the planting of two species of willow were effective and can be reproduced in similar conditions to those described in this study.
Título: Ex situ method and Salix spp. to treat polluted soil with hydrocarbon
Autor(es): Sabedot, Sydney; Bordignon, Sérgio Augusto de Loreto; Cunha, Ana Cristina Borda da
Resumo: This study shows the practices for managing ex situ soil with a simulation in which a residue derived from hydrocarbons with a sandy-silty soil was mixed, and two Salix spp. varieties were planted in the mixture. The experiment took place in a greenhouse and controls on plants and soil occurred during 36 months. The experiment aimed at two objectives, the first of which was to evaluate the development and resistance of the plants in the contaminated soil, comparing them with their planting in sandy-silty soil without the residue, and the second was to evaluate the performance of plants as phytoremediators of the residue. This study deals with the first goal. The success in sprouting and in development of the plants was verified in both types of soils. The diameter and height of the stems and the dimensions of the leaves were the measured parameters. Statistical analysis of the data indicated that Salix triandra species developed better. The joint result for both the above mentioned objectives indicated that the ex situ method for treating soils contaminated with hydrocarbons and the planting of two species of willow were effective and can be reproduced in similar conditions to those described in this study.2019-01-01T00:00:00ZLeishmanicidal and antichemotactic activities of icetexanes from Salvia uliginosa Benth
http://hdl.handle.net/11690/3030
Título: Leishmanicidal and antichemotactic activities of icetexanes from Salvia uliginosa Benth
Autor(es): Cezarotto, Caroll Schneider; Dorneles, Ariane; Baldissera, Fernanda Giesel; Silva, Matheus Brasil da; Markoski, Melissa M.; Rodrigues Júnior, Luiz C.; Peres, Alessandra; Fazolo, Tiago; Bordignon, Sergio Augusto de Loreto; Apel, Miriam Anders; Romão, Pedro Roosevelt Torres; von Poser, Gilsane Lino
Resumo: Background: Several species of Salvia are used as medicinal plants around the world. Biological activities of
isolated compounds have been described, being diterpenes frequently responsible for the effects.
Purpose: Isolation of diterpenes from Salvia uliginosa Benth. and evaluation of the antichemotactic and leishmanicidal activities of the isolated compounds.
Study design: To isolate diterpenes from S. uliginosa and evaluate their antichemotactic and leishmanicidal activities in vitro.
Methods: The exudate of S. uliginosa was obtained by rapidly dipping the aerial parts in dichloromethane. The
compounds were isolated by repeated column chromatography over silica gel. The effects on L. amazonensis
growth, survival, DNA degradation, ROS generation, as well as the antichemotactic activity and cytotoxicity of
the compounds towards human erythrocytes and macrophages were evaluated.
Results: A novel icetexane diterpene, isoicetexone (IsoICT) along with the known diterpenes icetexone (ICT), and
7-acetoxy-6,7-dihydroicetexone were isolated from the dichloromethane surface exudate of S. uliginosa. The
structures were elucidated using NMR and MS experiments, and by comparison with previously reported data.
IsoICT and ICT at low concentrations caused completely inhibition of neutrophils migration in vitro. In addition,
IsoICT and ICT showed high leishmanicidal activity against L. amazonensis, induced ROS production in parasites
and presented low cytotoxicity against macrophages and human erythrocytes, and moderate to high selectivity
index.
Conclusion: These data indicated that IsoICT and ICT exhibit potent antichemotactic and leishmanicidal effects.
Further studies are needed in order to evaluate the in vivo activities as well as the toxicity of the compounds.
Título: Leishmanicidal and antichemotactic activities of icetexanes from Salvia uliginosa Benth
Autor(es): Cezarotto, Caroll Schneider; Dorneles, Ariane; Baldissera, Fernanda Giesel; Silva, Matheus Brasil da; Markoski, Melissa M.; Rodrigues Júnior, Luiz C.; Peres, Alessandra; Fazolo, Tiago; Bordignon, Sergio Augusto de Loreto; Apel, Miriam Anders; Romão, Pedro Roosevelt Torres; von Poser, Gilsane Lino
Resumo: Background: Several species of Salvia are used as medicinal plants around the world. Biological activities of
isolated compounds have been described, being diterpenes frequently responsible for the effects.
Purpose: Isolation of diterpenes from Salvia uliginosa Benth. and evaluation of the antichemotactic and leishmanicidal activities of the isolated compounds.
Study design: To isolate diterpenes from S. uliginosa and evaluate their antichemotactic and leishmanicidal activities in vitro.
Methods: The exudate of S. uliginosa was obtained by rapidly dipping the aerial parts in dichloromethane. The
compounds were isolated by repeated column chromatography over silica gel. The effects on L. amazonensis
growth, survival, DNA degradation, ROS generation, as well as the antichemotactic activity and cytotoxicity of
the compounds towards human erythrocytes and macrophages were evaluated.
Results: A novel icetexane diterpene, isoicetexone (IsoICT) along with the known diterpenes icetexone (ICT), and
7-acetoxy-6,7-dihydroicetexone were isolated from the dichloromethane surface exudate of S. uliginosa. The
structures were elucidated using NMR and MS experiments, and by comparison with previously reported data.
IsoICT and ICT at low concentrations caused completely inhibition of neutrophils migration in vitro. In addition,
IsoICT and ICT showed high leishmanicidal activity against L. amazonensis, induced ROS production in parasites
and presented low cytotoxicity against macrophages and human erythrocytes, and moderate to high selectivity
index.
Conclusion: These data indicated that IsoICT and ICT exhibit potent antichemotactic and leishmanicidal effects.
Further studies are needed in order to evaluate the in vivo activities as well as the toxicity of the compounds.2019-01-01T00:00:00Z